Thermo Fisher Scientific Intern I in Carlsbad, California
- Since our lab offers PSC culture-training courses, all basic aspects of the basic ESC/iPSC workflow will be covered starting from iPSC generation to terminal differentiation.
LifeLab Stem Cell Training ( http://www.lifetechnologies.com/us/en/home/life-science/stem-cell-research/stem-cell-research-resources/stem-cell-training.html?cid=fl-we18153 )
Interns will be involved in feasibility experiments relevant to reprogramming and characterization. Some commonly used methods are listed: Somatic reprogramming of human cells using at least two different non-integrational methods in feeder-dependent and feeder-free media systems; Real-time visualization and measurement of reprogramming kinetics; Spontaneous and directed differentiation; Immunostaining, fluorescence microscopy and flow cytometry analysis; Cell-based and molecular analysis for iPSC characterization; Enrichment of differentiated cells
Optional: Involvement in projects focused on characterization platform for clinically relevant cell types, Gene editing/Knock-In using CRISPR and TALEN technology.
Additional Professional & Personal development opportunities include training in DOE (Design of Experiments), GLP/GMP workflows, record keeping, introduction to program management and product development, DiSC self-assessment, resume writing tips and mentorship program.
Thermo Fisher Scientific is an EEO/Affirmative Action Employer and does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability or any other legally protected status.